400 meter Women Semi Final, Heat:2 of 2 ; Official result since 15:44
| Rank | Lane | ID | Name | PB | Team | Cat | Result | Info | Video |
| 1 | 4 | 891 | COETZEE Miranda | 51.5 | RSA | Senior | 52.85 | Q | |
| 2 | 3 | 782 | SIMWAKA Asimenye | 52.9 | MAW | Senior | 54.28 | Q | |
| 3 | 7 | 612 | JELE Lydia | 53.40 | BOT | Senior | 54.42 | Q | |
| 4 | 6 | 843 | IDAMADUDU Praise | 54 | NGR | Senior | 54.44 | ||
| 5 | 5 | 610 | BASELE Thomphang | 54.09 | BOT | Senior | 55.90 | ||
| 6 | 8 | 934 | VILJOEN Marlie | 53.33 | RSA | Senior | 56.28 | ||
| 7 | 2 | 754 | SHIKANDA Jacinta | 53.97 | KEN | Senior | 56.78 | ||
| 8 | 1 | 700 | ZARAFO Amarech Zago | 54.28 | ETH | Senior | 59.04 |